Study on garlic oil combined with 5-FU induced apoptosis of adenoid cystic carcinoma cell line ACC-M.
- Author:
Fayin WU
;
Hefeng ZHOU
;
Zhiying FAN
;
Yawen ZHU
;
Yongye LI
;
Yukun YAO
;
Dan RAN
- Publication Type:Journal Article
- MeSH:
Allyl Compounds;
pharmacology;
Apoptosis;
drug effects;
Carcinoma, Adenoid Cystic;
pathology;
Cell Line, Tumor;
Fluorouracil;
pharmacology;
Humans;
Sulfides;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(3):175-181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of garlic oil combined with 5-FU induced apoptosis of adenoid cystic carcinoma cell line ACC-M.
METHOD:Human salivary in adenoid cystic carcinoma cell line AC-M was cultured, divided into the experimental group (5-FU group, garlic oil group, garlic oil + 5-FU group) and the control group, to observe the growth activity of tumor cells by MTT methods; to analyse the changes of cell cycle and apoptosis rate by flow cytometry.
RESULT:MTT experiments showed that 5-FU, garlic oil, garlic oil and 5-FU on ACC-M cells have inhibition in different concentration, with the increase of concentration and action time of the rise; Cell cycle analysis showed significant changes in flow cytometry. With the increase of concentration and the acting time, the G0/G1, phase of the cell ratio increased, S had no significant change, but G2/M phase cells decreased. Apoptosis rate display showed garlic oil combined with 5-FU induced apoptosis of ACC-M cells was significantly stronger than single group.
CONCLUSION:Garlic oil can effectively induce the apoptosis of adenoid cystic carcinoma cell line ACC-M. The effect of garlic oil combined with 5-FU on ACC-M cells was stronger than the garlic oil, 5-FU used alone.
