Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.
- Author:
Jing QING
;
Suping ZHAO
;
Weihong JIANG
;
Hua ZHANG
- Publication Type:Journal Article
- MeSH:
Artificial Gene Fusion;
methods;
Carcinoma;
Cell Line, Tumor;
Genes, Transgenic, Suicide;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(3):178-181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49.
METHOD:The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells.
RESULT:Suicide genes were expressed stably in CNE-2 cells.
CONCLUSION:We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.
