Expression of HIF-1alpha and VEGF in human laryngeal carcinoma and its relationship with angiogenes.
- Author:
Yashuang QIU
;
Huifang ZHOU
- Publication Type:Journal Article
- MeSH:
Female;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Laryngeal Neoplasms;
blood supply;
metabolism;
Larynx;
metabolism;
Male;
Microvessels;
Neovascularization, Pathologic;
etiology;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(6):389-393
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The aim of this study is to investigate the expression of hypoxia inducible factor-1alpha (HIF-1alpha), vascular endothelial growth factor (VEGF) in laryngeal carcinoma and its relations to clinical and pathophysiological characteristics, and determine the effect of HIF-1alpha, VEGF in the processing of angiogenesis. To offer the theoretical basis for the pathogenesis of laryngeal carcinoma and the new ideas for early diagnosis of laryngeal carcinoma.
METHOD:The major was divided into two groups:60 specimens of laryngeal carcinoma and 20 specimens of normal tissues beside the carcinoma. The expression of HIF-1alpha, VEGF was detected in 60 specimens of laryngeal carcinoma tissues and 20 specimens of normal tissues beside the carcinoma as controls by immunohistochemical staining technique. Microvessel was stained by antifactor CD105 antibody to analyse microvessel density.
RESULT:Positive rate of HIF-1alpha and VEGF protein expression was 71.67% (43/60) and 65.00% (39/60) respectively in laryngeal carcinoma tissues, which was significantly higher than that in normal laryngeal tissues (10.00% and 20.00%, respectively). HIF-1alpha expression was closely related to P-TNM stage, the grade of cell differentiation and lymph node status; VEGF expression was closely related to P-TNM stage and lymph node status. The coincidence rate of the expression of HIF-1alpha and VEGF was 56.67% (34/60), showing a close relation between them. MVD was much higher in tumor with HIF-1alpha(+)/VEGF(+) than that in tumor with HIF-1alpha(--)/VEGF(--) (P < 0.01).
CONCLUSION:HIF-1alpha protein and VEGF was over-expressed in laryngeal carcinoma. The positive expression of HIF-1alpha, VEGF and MVD in laryngeal carcinoma have a synergistic effect on the neovascularization of the tumor.
