Neoplasms stem cells play an important role in resistance of laryngeal squamous cancer to chemoradiotherapy.
- Author:
Rui HAN
;
Hui HUANGFU
;
Wei GAO
;
Chunming ZHANG
;
Yangzi JIN
;
Zhuo LI
;
Binquan WANG
- Publication Type:Journal Article
- MeSH:
AC133 Antigen;
Antibodies, Monoclonal, Humanized;
pharmacology;
Antigens, CD;
analysis;
Antineoplastic Agents;
pharmacology;
Apoptosis;
Cell Cycle;
Cell Line, Tumor;
Cetuximab;
Chemoradiotherapy;
Cisplatin;
pharmacology;
Drug Resistance, Neoplasm;
Flow Cytometry;
Glycoproteins;
analysis;
Humans;
Laryngeal Neoplasms;
therapy;
Neoplastic Stem Cells;
drug effects;
radiation effects;
Peptides;
analysis;
Radiation Tolerance
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(6):400-405
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine an approach enriching cancer stem cells from laryngeal cancer cell line. To investigate whether laryngeal cancer stem cells in chemoradiotherapy have the characteristic of resistance.
METHOD:CD133+ cells and CD133- cells was detected and isolated from Hep-2 cell line by fluorescence activated cell sorting technology. The cytotoxicities of cisplatin and radiation were investigated by cell counting kit-8(CCK-8) assay. The apoptosis and cell cycle was analyzed with flow cytometry.
RESULT:CD133+ cells accounted for a fraction of (2.43 +/- 0.77)% in Hep-2 cell line. CD133+ cells have a more obvious characteristics of cancer stem cells. Different cisplatin and radiation concentrations of for two cell have inhibition, in a certain concentration range and the dosage dependence. Cisplatin and radiation had synergistic inhibitory effects with CD133- cells on the growth of two cell. Moreover, cell cycle arrest at G0/G1 phase and more apoptosis was induced by synergistic combination. Different concentrations of cetuximab for Hep-2 cells have inhibition, in a certain concentration range and time and the dosage dependence. The half maxial inhibitory concentration (IC50) of cetuximab to Hep-2 cells on 24 h was 1 036.84 microg/L. Cisplatin and radiation had synergistic inhibitory effects with cetuximab on the growth of Hep-2 cell line. Moreover, cell cycle arrest at G0/G1 phase and more apoptosis was induced by synergistic combination.
CONCLUSION:Compared with CD133- cells, CD133+ cells subpopulation exhibited extraordinary cancer stem.
