Anti-allergic effects of xuebijing and potential role of heme oxygenase-1 against ovalbumin-induced murine allergic rhinitis model.
- Author:
Dongmei SONG
1
;
Yinghao NIU
;
Jiantao WANG
;
Jing XUE
;
Xin LV
;
Jianwang YANG
;
Baoshan WANG
Author Information
1. Department of Otolaryngology Head and Neck Surgery, Devision of Allergic Medicine, First Hospital of Hebei Medical University, Shijiazhuang, 050031, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
pharmacology;
therapeutic use;
Eosinophilia;
metabolism;
Heme Oxygenase-1;
metabolism;
Immunoglobulin E;
immunology;
Inflammation;
drug therapy;
metabolism;
Interleukin-13;
metabolism;
Interleukin-4;
metabolism;
Interleukin-5;
metabolism;
Membrane Proteins;
metabolism;
Mice;
Nasal Mucosa;
metabolism;
Rhinitis, Allergic;
Rhinitis, Allergic, Perennial;
chemically induced;
drug therapy;
metabolism;
Tumor Necrosis Factor-alpha;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(16):899-904
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:In this study, we investigated the anti-inflammation effects of Xuebijing in OVA-induced murine allergic rhinitis model. Furthermore, we determined whether heme oxygenase (HO)-1 is required for the protective activity of Xuebijing.
METHOD:Airways of OVA-sensitized mice exposed to OVA challenge developed eosinophilia, mucus hypersecretion and increased cytokine levels. Levels of interleukin IL-4, IL-5, IL-13, and tumor necrosis factor (TNF)-alpha in nasal lavage fluid were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue and nasal mucosa sections were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration and mucus production, Immunohistochemistry, Real-time PCR and Western Blot analyses for HO-1 protein expression.
RESULT:Orally administered Xuebijing significantly inhibited the number of OVA-induced inflammatory cells and IgE production, along with reduced T-helper (Th) 2 cytokine levels, such as IL-4, IL-5 and IL-13, improved the level of IFN-gamma, in nasal lavage fluid. In addition, Xuebijing induced a marked decrease in OVA-induced inflammatory cell infiltration and mucus production in nasal and lung tissues. These effects were correlated with HO-1 mRNA and protein induction.
CONCLUSION:Our results indicate that Xuebijing protects against OVA-induced airway inflammation, at least in part, via HO-1 upregulation.
