Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4.
- Author:
Genyu LUO
1
;
Rui GONG
2
;
Zhenkui GONG
1
;
Hua LI
1
;
Changyong ZOU
1
;
Rongfei ZHU
3
;
Jianjun CHEN
4
Author Information
1. Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
2. Hubei Extny Exit Inspection and Quarantine Bureau.
3. Department of Allergy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology.
4. Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology.
- Publication Type:Journal Article
- MeSH:
Animals;
Calibration;
Enzyme-Linked Immunosorbent Assay;
methods;
Humans;
Immunoglobulin G;
blood;
Indicators and Reagents;
Pyroglyphidae;
immunology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(17):921-924
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.
METHOD:The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.
RESULT:The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.
CONCLUSION:The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
