Effect of MAPK/NF-kappaB signaling pathway on extracellular release of HMGB1 induced by hypoxia in laryngeal Hep-2 carcinoma cells.
- Author:
Lei LI
1
;
Xiabing TANG
2
;
Falong WANG
3
;
Feifei HAN
1
;
Weidong ZHOU
2
;
Guoqian CHEN
1
Author Information
1. Department of Medical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, 214023, China.
2. Department of Otolaryngology, Wuxi People's Hospital Affiliated to Nanjing Medical University.
3. Department of Medical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, 214023, China
- Publication Type:Journal Article
- MeSH:
Anthracenes;
Cell Hypoxia;
Cell Line, Tumor;
Flavonoids;
HMGB1 Protein;
metabolism;
Humans;
Imidazoles;
MAP Kinase Signaling System;
NF-kappa B;
antagonists & inhibitors;
metabolism;
Protein Kinase Inhibitors;
Pyridines;
Pyrrolidines;
RNA, Messenger;
genetics;
Thiocarbamates
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(19):1076-1079
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the extracellular release of high mobility group box 1 (HMGB1) in laryngeal Hep-2 carcinoma cells induced by hypoxia and its possible mechanism.
METHOD:The changes of HMGB1 concentration in the culture medium as well as HMGB1 protein and mRNA expression in Hep-2 cells were investigated after the cells were cultured with 1% O2 for different durations. Inhibitory effects of MAPK pathway inhibitors (PD98059. SP600125, and SB202190) and nuclear NF-kappaB pathway inhibitor (PDTC) with various concentrations on extracellular HMGB1 release were observed in hypoxia-induced Hep-2 cells. The HMGB1 concentration and HMGB1 protein expression were measured by enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. The HMGB1 mRNA expression was determined by real-time quantitative PCR(RT-PCR).
RESULT:The HMGB1 concentration in the culture medium and the HMGB1 protein expression in Hep-2 cells increased after the cells were subjected to hypoxia culture for 12 h in a time-dependent manner. The level of HMGB1 mRNA expression in Hep-2 cells increased after the cells were induced by hypoxia for 6h PD98059 and SP600125 with 20 micromol/ L and PDTC with 50 mg/L partly inhibited extracellular release of HMGB1 in hypoxia-cultured Hcp-2 cells.
CONCLUSION:Hypoxia induces laryngeal carcinoma cells to release HMGH1. which may be related to MAPK/NF-kappaB signaling pathway.
