Expression and clinical significance of stromelysin-3 in laryngeal cancer.
- Author:
Shihong DUAN
1
;
Yufen GUO
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Second Hospital, Lanzhou University, Lanzhou, 730030, China. duanqiushuo@163.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Biomarkers, Tumor;
Female;
Humans;
Laryngeal Neoplasms;
metabolism;
pathology;
Lymphatic Metastasis;
Male;
Matrix Metalloproteinase 11;
metabolism;
Middle Aged;
Neoplasm Invasiveness
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(3):104-107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of expression of stromelysin-3 in laryngeal carcinoma and the relationship between stromelysin-3 expression and the invasion and metastasis of laryngeal cancer.
METHOD:The expression of stromelysin-3 in 63 samples with laryngeal carcinoma and 63 adjacent normal tissue were detected by immunohistochemistry. The mRNA expression of stromelysin-3 were examined by reverse transcript-polymerase chain reaction (RT-PCR) in 22 frozen specimens of laryngeal cancer and 22 adjacent normal tissue.
RESULT:The immunochemistry showed the expression of stromelysin-3 in laryngeal cancer is much higher than that in adjacent normal tissue (P < 0.01), RT-PCR showed the expression of stromelysin-3 in laryngeal cancer was much higher than that in adjacent normal tissue (P < 0.01). The expression of stromelysin-3 in protein level was not only much higher in T3 + T4 group than that in T1 + T2 group (P < 0.01) but also much higher in nodal metastases group than that in the group without nodal metastases (P < 0.05). The mRNA expression of stromelysin-3 was higher in T3 +T4 group than that in T1 + T2 group (P < 0.05) and was higher in nodal metastases group than that in group without nodal metastases (P < 0.05). The expression of stromelysin-3 both in protein level and in mRNA level was not associated with different primary location and different histological grade of laryngeal cancer (P > 0.05).
CONCLUSION:The expression of stromelysin-3 was closely associated with the invasion and metastases of laryngeal carcinoma, it may be served as a marker in estimating the invasion and metastases potency of laryngeal cancer.
