Effect of interferon-gamma on the expression of vascular endothelial growth factor C on Hep-2 laryngeal carcinoma cell lines.
- Author:
Jie CHEN
1
;
Jiaqing ZHOU
;
Jiadong WANG
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Shanghai Jiaotong University, Shanghai, 200001, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Humans;
Interferon-gamma;
pharmacology;
Laryngeal Neoplasms;
metabolism;
pathology;
Vascular Endothelial Growth Factor C;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(3):108-111
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of interferon-gamma on the expression of vascular endothelial growth factor C on Hep-2 laryngeal carcinoma cell lines.
METHOD:Hep-2 cell was exposed to interferon-gamma at a concentration (10(3), 10(4), 10(5), 10(6), 10(7) U/L) for 0, 12, 24, 36, 48, 60, 72 h hours. MTT colorimetric assay was used to evaluate the effect of interferon-gamma on Hep-2 cell proliferation after incubation with interferon-gamma. Hep-2 cell was harvested to be detected VEGF-C mRNA levels by realtime polymerase chain reaction (Realtime-PCR) with interferon-gamma (10(6) U/L) for 0, 2, 6, 12, 24, 36, 48, 60, 72 h hours. The VEGF-C protein level in supernatants was determined by enzyme-linked immunosorbent assay (ELISA) and in cytoplasma by immunocytochemical staining and Western blot analysis with the same drug treatment as Realtime-PCR.
RESULT:Hep-2 cell was significantly inhibited by IFN-gamma at high concentration. Expression of VEGF-C mRNA and protein levels of Hep-2 cell at 10(6) U/L concentrations of interferon-gamma were lower than that of normal control (P < 0.05) at 48 h.
CONCLUSION:IFN-gamma can inhibit the expression of VEGF-C in Hep-2 cell with the level of mRNA and protein.
