Effect of inhibitor HNP1 transfection on tumor growth of human nasopharyngeal carcinoma cell line HNE-1.
- Author:
Hongbin YANG
1
;
Gang QIN
;
Zumao LI
;
Ning XU
;
Yanjun WEN
;
Chuanyu LIANG
Author Information
1. Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu, 610041, China. hongbiny@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Female;
Humans;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Nasopharyngeal Neoplasms;
genetics;
pathology;
Transfection;
Tumor Burden;
alpha-Defensins;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(3):124-137
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The aim of this study is to investigate the effects of inhibitor HNP1 transfection on proliferation and tumor growth of human nasopharyngeal carcinoma cell line HNE1.
METHOD:HNE1 cells were transfected with HNP1 by liposome, and the cytotoxic effect was detected by MTT, In vivo tumor growth test was performed in nude mice inoculated with transfected HNE1 cells. The therapeutic effect of HNP1 was evaluated in the inoculated tumors, alpha-defensin 1 expression was detected in implanted tumor tissues by immunohistochemical stain.
RESULT:HNP1 transfection significantly inhibited the proliferation of HNE1 cells. MTT assay confirmed cytotoxic effect. In vivo study showed tumor volume was significantly smaller in HNP1 transfection group than that in control group (P < 0.01). Immunohistochemical stain showed alpha-defensin expression was increased in HNP1 transfection group.
CONCLUSION:HNP1 transfection can inhibit the proliferation of HNE1 cells, as well as tumor growth.
