Expression and distribution of monocyte chemotactic protein-3 and its significance in laryngeal carcinoma.
- Author:
Lin LI
1
;
Dongdong ZHU
;
Zhen DONG
Author Information
1. Department of Otolaryngology Head and Neck Surgery, China-Japan Friendship Hospital, Jilin University, Jilin, 130033, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
metabolism;
pathology;
Chemokine CCL7;
metabolism;
Female;
Humans;
Laryngeal Neoplasms;
metabolism;
pathology;
Male;
Middle Aged;
RNA, Messenger;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(6):244-247
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate monocyte chemotactic protein-3 (MCP-3) mRNA and protein expression and distribution in laryngeal squamous cell carcinoma (LSCC) tissue, so as to study its function in the tumor development.
METHOD:Immunohistochemical staining and RT-PCR, and western blot were performed to detect the LSCC tissue and the normal tissue adjacent tumor.
RESULT:The expression of MCP-3 mRNA and protein were found in the LSCC tissue and the normal tissue adjacent tumor. The expression of MCP-3 mRNA and protein in LSCC tissue is much higher than that in normal tissue adjacent tumor (P<0.05). There is a significant difference between I-II stage III-IV stage (P<0.05). Immunohistochemical staining: positive staining of MCP-3 was yellow and mainly detected in the tumor cell cytoplasm and also in some normal glandular epitheliums.
CONCLUSION:MCP-3 might be involved in the mechanism of the tumor development, its function of chemotaxis and activation of the tumor-associated macrophages would be highlighted, so that the inflammatory microenvironment might play an important role in the development of tumor.
