Immunohistochemical Study on the Expression of c-erbB-2 Oncoprotein in the Odontogenic cysts.
- Author:
Sung Woon PYO
1
;
Chang Hyen KIM
;
Won LEE
;
Young Sil KIM
Author Information
1. Divison of Oral & Maxillofacial Surgery, Dept. of Pathology.
- Publication Type:Original Article
- Keywords:
odontogenic cyst;
immunohistochemistry;
c-erbB-2 oncoprotein
- MeSH:
Dental Sac;
Epithelial Cells;
Formaldehyde;
Immunohistochemistry;
Odontogenic Cysts*;
Peroxidase
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2001;27(1):32-36
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To investigate epithelial cell proliferation reactivity in the odontogenic cysts, the expression of c-erbB-2 oncoprotein by epithelial lining was studied in odontogenic keratocyst(OKC, n=10), dentigerous cyst(DC, n=12), radicular cyst(RC, n=12) and normal dental follicle(n=7). The c-erbB-2 immunoreactivity was studied using a streptavidine- biotin- peroxidase method with polyclonal rabbit anti-human antibody to c-erbB-2 oncoprotein which is known to react with formalin fixed, paraffin-embedded sections and the intensity of staining was determined by manually. In all of 10(100%) OKCs, showed positive expression for c-erbB-2 oncoprotein compared with 10/12(83.3%) in DCs, 11/12(91.7%) in RCs and 5/7(71.4%) in normal dental follicles. The expression within OKC was higher than that of DC, RC and dental follicle but statistically not significant(p>0.05) and but may reflects underlying genetic defect. These results demonstrate differences in c-erbB-2 expression between the epithelial linings of the three major odontogenic cyst types, indicating differences in proliferation activity and differentiation processes within these lesions. And, in particular, these results are able to explain the peculiar aggressive growth pattern of OKC.
