Inhibiting effects of TFPI-2 recombinant adenovirus on invasion of laryngeal squamous carcinoma.
- Author:
Ya'nan SUN
1
;
Ming LIU
;
Yuli XIAO
;
Linli TIAN
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Animals;
Carcinoma, Squamous Cell;
pathology;
Cell Line, Tumor;
Genetic Therapy;
Genetic Vectors;
Glycoproteins;
genetics;
Humans;
Laryngeal Neoplasms;
pathology;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(7):315-317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of adenovirus-mediated tissue factor pathway inhibitor-2 gene on the invasion of laryngeal squamous cancer.
METHOD:Ad-TFPI-2 was transfected into laryngeal squamous cancer (Hep-2) cell. Western-blot was used to test the TFPI-2 protein expression and Boyden Chamber experiment was used to examine the invasive ability of Hep-2 cells. Furthermore, the Ad-TFPI-2 infected Hep-2 cells were subcutaneously inoculated in nude mice and the tumor formation capability were observed.
RESULT:Ad-TFPI-2 was identified correctly by endonuclease and sequencing and the virus titer was 2.8 x 10(13) PFU/L. In the Hep-2 cells of treated group, the TFPI-2 protein expression was increased while the invasive capability was descent. The tumor formation capability was also decreased in the treated group nude mouse model.
CONCLUSION:TFPI-2 recombinant adenovirus can effectively inhibit the invasive capability of laryngeal squamous cancer.
