Expression of TLR9 in human nasal epithelial cells.
- Author:
Xuewei ZHU
1
;
Dongdong ZHU
;
Zhen DONG
;
Kewei SUN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun, 130033, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Epithelial Cells;
metabolism;
Humans;
Nasal Mucosa;
cytology;
metabolism;
RNA, Messenger;
genetics;
Toll-Like Receptor 9;
genetics;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(8):356-358
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze expression of TLR9 in human nasal epithelial cells by semi-quantitative reverse transcription-polymerase chain reaction, immunohistochemistry and flow cytometry.
METHOD:Human primary nasal epithelial cells (HNECs) were cultured, and then analyze the expression of TLR9 in primary cultured HNECs measured by semi-quantitative RT-PCR and immunohistochemistry and flow cytometry.
RESULT:Primary cultured HNECs were observed by 400 x optical microscopes. Round or irregular cells stick to the bottom of cell culture plates. RT-PCR showed that mRNA expressions of TLR9 were found in primary nasal epithelial cells by 1% agarose gel electrophoresis. Interestingly, by analyzing with gray level of GAPDH, expression of TLR9 mRNA in HNECs was higher than positive control PBMCs.
CONCLUSION:TLR9 is expressed by HNECs. Expression of TLR9 mRNA in nasal epithelial cells is higher than in PBMCs.
