Inhibition of growth and proliferation of Hep-2 cells by targeting c-myc gene using small interference RNA technology.
- Author:
Zhi TANG
1
;
Jiqun WANG
;
Xinyi WU
;
Tao ZHANG
;
Xueli LIN
;
Yanchun SHAN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Shunde, Foshan, 528300, China. damaodoctor@126.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Division;
Cell Line, Tumor;
Cell Proliferation;
Gene Targeting;
Genes, myc;
genetics;
Humans;
RNA, Small Interfering;
genetics;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(9):399-402
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.
METHOD:siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.
RESULT:1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.
CONCLUSION:siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
