Effects of siRNA targeting NF-kappaBp65 against in proliferation of Hep-2 cells and the expression of mRNA and protein of NF-kappaBp65.
- Author:
Hongjun JIN
1
;
Weihua LOU
;
Jianzhong SANG
;
Yamin ZHANG
Author Information
1. Department of Otorhinolaryngology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation;
Humans;
RNA, Messenger;
genetics;
RNA, Small Interfering;
Transcription Factor RelA;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(9):416-418
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study siRNA inhibited the expression of mRNA and protein of NF-kappaBp65 in the Hep -2 cell line.
METHOD:Hep-2 were transfected with p65SiRNA. Western blotting was used to examine the protein levels of NF-kappaBp65. RT-PCR method was adopted to determine the mRNA expression of NF-kappaBp65. MTT method was adopted to investigate the proliferation of the Hep-2 cells after the transfection of p65siRNA.
RESULT:The western blotting result showed that the level of NF-kappaBp65 protein was gradually declined after transfection of p65siRNA. The RT-PCR result showed that transfection with p65siRNA caused special degradation of the p65mRNA in Hep-2 cells at 24, 48 and 72 h. After transfection with p65siRNA.
CONCLUSION:p65siRNA has significant inhibition effects on the proliferation of the Hep-2 cells and expression of purpose gene mRNA and protein. The inhibition effects are time depended.
