Gene expression profiles in human nasal polyps studied by DNA microarray.
- Author:
Bing LIU
1
;
Jian WU
;
Jingping FAN
;
Yucheng PENG
Author Information
1. Department of Otorhinolaryngology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Female;
Gene Expression;
Gene Expression Profiling;
Humans;
Male;
Middle Aged;
Nasal Polyps;
genetics;
Oligonucleotide Array Sequence Analysis;
Reverse Transcriptase Polymerase Chain Reaction;
Young Adult
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(11):495-497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the changes in gene expressions in the human nasal polyp.
METHOD:A 14500 gerie DNA microarray (Affymetrix) was used to examine gene expressions in 6 NP samples, 6 normal mucosal samples. The differentially expressed genes were identified and subjected to realtime PCR analysis.
RESULTS:The differentially expressed genes mostly involved in cytokines, adhesion genes about complements and their receptors, immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8, RGS1, GRK4, CCL20, uteroglobin. The results of IL-8, RGS1 Real-time PCR of were consistent with that of gene chip analysis.
CONCLUSION:Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.
