The expression of COX-2 mRNA and protein in human laryngeal squamous cell carcinoma.
- Author:
Wangyan CHEN
1
;
Gang DENG
;
Qi YAO
;
Chengzhang YANG
;
Bo LIU
Author Information
1. Department of Otorhinolaryngology, Chinese and Western Medicine Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
genetics;
metabolism;
pathology;
Cyclooxygenase 2;
genetics;
metabolism;
Female;
Humans;
Laryngeal Neoplasms;
genetics;
metabolism;
pathology;
Male;
Middle Aged;
RNA, Messenger;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(12):532-535
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis.
METHOD:By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined.
RESULT:Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased (P < 0.01). The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed.
CONCLUSION:COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemistry is sensitive and specific for laryngeal neoplasms detection.
