The value of loop-mediated isothermal amplification method for rapid diagnosis of EBV DNA.
- Author:
Guohui NIE
1
;
Hongsong DONG
;
Guihua HE
;
Xiaoping XU
;
Lei SHI
;
Yicheng CAO
;
Xun CHEN
Author Information
1. Department of Otolaryngology, Second People's Hospital of Shenzhen, Shenzhen, 518035, China. nghui@21cn.com
- Publication Type:Journal Article
- MeSH:
DNA Primers;
DNA, Viral;
analysis;
Herpesvirus 4, Human;
genetics;
Humans;
Molecular Sequence Data;
Nasopharyngeal Neoplasms;
diagnosis;
Nucleic Acid Amplification Techniques;
Sensitivity and Specificity
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(12):555-557
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a rapid method for EBV detection with loop-mediated isothermal amplification (LAMP), and to make it as a clue for early diagnosis of nasopharyngeal carcinoma cancer.
METHOD:EBV DNA was fast extracted from samples after boiling, while the whole detection will be finished within an hour with specific amplification of EBV gene by LAMP.
RESULT:High specificity was shown from EBV detection of 33 clinical samples. Comparing with PCR, LAMP is more simple and convenient to perform under isothermal conditions, and require no special apparatus, thus, it is more economical and practical.
CONCLUSION:LAMP analysis of EBV may be an efficient and easy way for clinical diagnosis of nasopharyngeal carcinoma.
