Expression and significance of Notch receptors in the mouse model of allergic rhinitis.
- Author:
Guoxi ZHENG
;
Liangliang LIU
;
Kang ZHU
;
Junrong WEI
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Leukocytes, Mononuclear;
Mice;
Mice, Inbred BALB C;
Nasal Mucosa;
RNA, Messenger;
Receptors, Notch;
metabolism;
Rhinitis, Allergic;
metabolism;
Rhinitis, Allergic, Perennial
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(20):1585-1589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and significance of related receptors of Notch signaling pathway in mouse model of allergic rhinitis (AR).
METHOD:Sixteen BALB/c mice of seven-eight weeks old were randomly assigned to two groups,including controls group and model group. AR model mice was sensitized with ovalbumin(OVA). Symptom score, hematoxylin-eosin for pathological alteration and infiltration of inflammatory cells in nasal mucosa were analyzed as well as enzyme linked immunosorbent assay was taken to detect IgE in pe- ripheral serum. Nasal septum mucosa and peripheral blood mononuclear cells were collected from 16 BALB/c mouse(8 Allegic rhinitis,8 controls). Notch 1-4 were checked by real-time quantitative polymerase chain reaction and flow cytometry from different levels.
RESULT:BALB/c mice model of allergic rhinitis was established successfully. The mRNA of Notch1, Notch3, Notch4 in nasal septum mucosa of allergic rhinitis mice model groups were obviously higher than that in normal controls, and the difference were statistically significant (P < 0.01). However, The expression of Notch2 is lower than the control group and the difference was statistically significant (P < 0.05). In line with the above, the protein expression of Notch1, Notch3, Notch4 in peripheral blood mononuclear cells (PBMC) of model groups were significantly higher than that in health controls, and the difference were statistically significant (P < 0.01). But comparing control, expression of Notch2 was lower and the difference was statistically significant (P < 0.05).
CONCLUSION:There were significant changes of Notch genes in mouse model of AR. This intimated that related genes of Notch signaling pathway may paly important roles in the development and progression of AR and provide ideas for in depth study of the pathogenesis of AR.
