Combined application the siRNA target for Hif-lα and Survivin gene to human NPC CNE-2 cell and its effects on the proliferation and cycle of this cell.
- Author:
Yanxin REN
;
Shuping ZHANG
;
Xiaojiang LI
;
Jie YANG
;
Ruimei SUN
;
Liufang ZHAO
;
Lei LI
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Carcinoma;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
genetics;
Inhibitor of Apoptosis Proteins;
genetics;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
genetics;
Plasmids;
RNA, Messenger;
RNA, Small Interfering;
Survivin;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(24):1975-1978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the combined transfection of the siRNA target for Hif-lα and Survivin gene to human NPC CNE-2 cell and its effects on the proliferation and cycle of this cell.
METHOD:Combined transfection of the siRNA target for Hif-lα and Survivin gene to human NPC CNE-2 cell, these plasmids were respectively transfected into the same cells. Cell proliferation was detected with MTT assay. The inhibitory effects on target genes were evaluated with RT-PCR and Western Blot at the levels of mRNA and protein, respectively.
RESULT:MTT showed that CNE-2 cell proliferation in multi-gene plasmid group was more significantly inhibited than a single gene. The expression of mRNA and protein of two different genes were both decreased in HS group, and the interference effect of multiple genes was better than the single-gene(P<0.01).
CONCLUSION:HS group could restrain cell proliferation and interference the mRNA and protein expression in nasopharyngeal carcinoma CNE-2 cell, which was better than the other groups.
