Study on delivery efficiency and cytotoxicity of Hela cells with mPEG-PLGA-BSA-FITC-NPs nanocarrier.
- Author:
Zhiting CHEN
;
Nan WU
;
Xiongwei DENG
;
Fangyuan WANG
;
Kun LI
;
Weiwei GUO
;
Riyuan LIU
;
Shuolong YUAN
;
Jiakun ZHANG
;
Shiming YANG
- Publication Type:Journal Article
- MeSH:
Drug Carriers;
Fluorescein-5-isothiocyanate;
analogs & derivatives;
HeLa Cells;
Humans;
Nanoparticles;
Particle Size;
Polyesters;
Polyethylene Glycols;
Serum Albumin, Bovine
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(1):48-56
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct and obtain ideal protein delivery vectors by researching the delivery efficiency and cytotoxicity to Hela cells using mPEG-PLGA-BSA-FITC-NPs.
METHOD:The mPEG-PLGA nanoparticle was obtained through surface modification of PLGA with PEG, and deliver BSA-FITC into Hela cells in vitro. The positive cells were counted by Laser scanning confocal microscopy and the survival rate of Hela cells was calculated by MTT assay at different time points.
RESULT:mPEG-PLGA-BSA-FITC-NPs shows the classic nanometer size, and the encapsulation efficiency reached 51. 2%. At the same time, the nanoparticles possess characteristics of slow release. By optimizing the delivery conditions, the highest efficiency of mPEG-PLGA-BSA-FITC-NPs was above 65.2%, and the cellular viability was about 85.7%.
CONCLUSION:mPEG-PLGA-BSA-FITC-NPs nanoparticles can successfully carry the target protein into cells as safe and effective as novel delivery materials of protein in vitro, and has shown slow release characteristics. The mPEG-PLGA-BSA-FITC-NPs provide ideal delivery vector for future application in clinical treatment of disease using nano-materials.
