Expression of cancer-testis antigen SCP-1 mRNA in human nasopharyngeal carcinoma.
- Author:
Min YI
1
;
Hong LIAO
;
Shaoming HUANG
;
Xiaoxun XIE
;
Guorong LUO
Author Information
1. Department of Histology and Embryology, Guangxi Medical University, Nanning, 530021, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Antigens, Neoplasm;
genetics;
metabolism;
Carcinoma, Squamous Cell;
genetics;
immunology;
metabolism;
Case-Control Studies;
Female;
Humans;
Male;
Nasopharyngeal Neoplasms;
genetics;
immunology;
metabolism;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2007;21(8):343-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the gene expression of Cancer-Testis Antigen SCP-1 in human nasopharyngeal carcinoma.
METHOD:The SCP-1 mRNA were examined in the tumor tissues of 28 nasopharyngeal carcinoma cases and control pharynx vasitis tissues of 40 non-cancer cases by reverse transcriptase polymerase chain reaction. A randomized sample of positive expression of SCP-1 mRNA was extracted for DNA sequencing to examine the reliability of results. The correlation of the expression of SCP-1 mRNA with clinical data was analyzed by statistical method.
RESULT:The expression frequency of SCP-1 mRNA was 14.3% (4/28) in nasopharyngeal carcinoma and zero (0/40) in control pharynx vasitis tissue of non-cancer cases (P < 0.05); The DNA sequencing confirmed that the product of reverse transcriptase polymerase chain reaction was truly the target gene; No significant relationship was found between the expression of SCP-1 mRNA and clinical characters, such as age, gender and serum anti-EBV level (P > 0.05).
CONCLUSION:SCP-1 mRNA is expressed in some nasopharyngeal carcinoma, suggesting that SCP-1 might be a new tumor target antigen of nasopharyngeal carcinoma.
