Detection of cytokeratin18 and cytokeratin19 gene expression in blood and tumor tissue of nasopharyngeal carcinoma patients by RT-PCR.
- Author:
Shuo HUANG
;
Shuang LI
;
Tao PENG
;
Tingting WU
;
Peng SONG
;
Xuhong ZHOU
- Publication Type:Journal Article
- MeSH:
Biomarkers, Tumor;
Carcinoma;
DNA, Complementary;
Gene Expression;
Humans;
Keratin-18;
biosynthesis;
Keratin-19;
biosynthesis;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
diagnosis;
metabolism;
pathology;
Neoplasm Metastasis;
Prognosis;
RNA, Messenger;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(2):111-116
- CountryChina
- Language:Chinese
-
Abstract:
UNLABELLED:Abstract
OBJECTIVE:To measure the expression of CK18 and CK19 in the cells from peripheral blood and tumor tissue of the nasopharyngeal carcinoma patients,to test whether CK 18 and CK 19 could be biomarkers of nasopharyngeal carcinoma fordiagnosis.
METHOD:The mRNA was extracted from the blood and carcinoma tissue of nasopharyngeal carcinoma and was reversed transcription to cDNA. The 3 pairs primers were designed for RT-PCR and the fold value was calculated to evaluated expression by ΔCT.
RESULT:There are no statistical differences between the CK18 and CK19 gene expression and the gender, age and metastasis in tumor tissue of 45 nasopharyngeal carcinoma patients (P>0. 05). There are significant differences among 3 pathological stages and 2 genes expressed increase as the grade malignancy (P<0. 05). The detecting of the 2 genes expression from blood cells shows that CK18 and CK19 had a high positive ratio 64% and 75% respectively. Meanwhile this method showed a same detection characteristic in tumor and blood, the positive.rate of CK18 and CK19 genes in metastasis is higher than non-metastasis. The results showed CK18 has a high specificity and CK19 has a high sensitivity for prognosis and all relapsed cases are associated with the expression of CK18 and CK19.
CONCLUSION:CK18 and CK19 may be used as biomarkers of nasopharyngeal carcinoma for diagnosis.
