The vitro research of effects of Beclin1 on paclitaxel-sensitivity in laryngeal carcinoma cell Hep-2.
- Author:
Xiaocong DENG
;
Xinming YANG
;
Shisheng LI
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Apoptosis Regulatory Proteins;
physiology;
Beclin-1;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Laryngeal Neoplasms;
pathology;
Larynx;
Membrane Proteins;
physiology;
Paclitaxel;
pharmacology;
Phosphatidylinositol 3-Kinases
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(2):159-163
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Background: We detect the effects of Beclinl on paclitaxel-sensitivity in laryngeal carcinoma cell.
METHOD:This study used Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl as invitro model. The effect of paclitaxel on the proliferation and cell apoptosis of laryngeal cancer cell lines was evaluated by MTT assay and flow cytometry. The protein expression level of Akt and p-Akt was detected by Western blot. Result: After treated by paclitaxel, the inhibition rate was significantly higher in Hep-2-Beclin cells than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<. 05). After dealing with 10 tg/L paclitaxel, the apoptosis rate in Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl were (23. 75 ± 2 3. 77) %, (21. 25 ± 4. 92) %, (32. 50 ± 5. 97) %, respectively. After dealing with 20µg/L paclitaxel, the apoptosis rate in Hep-2, Hep-2-pcDNA3. 1, Hep-2-Beclinl were (38. 75 ± 4. 79) %, (38. 75±6. 55) %, (50. 00±7. 26) %, respectively. Paclitaxel-induced apoptosis was higher in Hep-2-Beclin cells than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<. 05). The result of western blot showed that the protein expression level of p-Akt in Hep-2-Beclin cells was lower than in Hep-2-pcDNA3. 1 cells and Hep-2 cells (P<0. 05) and the protein expression level of Akt was similar in three cell lines (P>0. 05).
CONCLUSION:Beclinl enhances paclitaxel-sensitivity by inhibition of PI3K/Akt pathway.
