Expression of AMCase mRNA in nasal mucosa of allergic rhinitis in SD rats.
- Author:
Yan LIU
;
Zhimin XING
;
Min WANG
- Publication Type:Journal Article
- MeSH:
Animals;
Chitinases;
metabolism;
Nasal Mucosa;
metabolism;
Ovalbumin;
RNA, Messenger;
metabolism;
Rats;
Rats, Sprague-Dawley;
Rhinitis, Allergic;
enzymology;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2015;29(3):267-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine AMCase mRNA expression levels in nasal mucosa of allergic rhinitis SD rats.
METHOD:Thirty SD rats were chosen and randomly divided into the experimental group and the control group. 20 in experimental group and 10 in the control group. AR model rats were established through repeated intraperitoneal shot of ovalbumin (OVA) for 2 weeks and consequently confirmed by local challenge with OVA for 1 week. The control group was treated by the same method with Physiological saline water instead of OVA. After the last excitation allergic rhinitis was diagnosed according to the accumulation score about nasal symptom. The septal mucosa of all rats were used to diagnose pathologically by HE dyeing. AMCase mRNA in nasal mucosa, obtained from the bilateral nasal mucosa in two groups, were used to do reverse transcriptive polymerase chain reaction. Real-time polymerase chain reaction was used to examine AMCase mRNA expression levels.
RESULT:(1) The results showed definitely that there were positive expression of AMCase mRNA in normal nasal mucosa. This expression increase significantly during nasal allergy (P < 0.05). (2) The increased expression of AMCase mRNA in allergic rhinitis are related with the nasal symptoms score (r = 0.411, P < 0.05).
CONCLUSION:Increased expression of AMCase mRNA in nasal mucosa in allergic rhinitis model might play roles in the pathogenesis of AR, and Restrain the enzyme activity could become new treatment targets of allergic rhinitis.
