Effect of clarithromycin on the expressions of cyclooxygenase-2 and nuclear factor-kappa B in nasal polyps in vitro.
- Author:
Mingxia ZHANG
1
;
Yuan LI
;
Zhenlin WANG
;
Peng LI
;
Gehua ZHANG
;
Qiuhang ZHANG
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Clarithromycin;
pharmacology;
Cyclooxygenase 2;
metabolism;
Female;
Humans;
In Vitro Techniques;
Male;
Middle Aged;
NF-kappa B p50 Subunit;
metabolism;
Nasal Polyps;
metabolism;
Transcription Factor RelA;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(17):769-772
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the expression and correlation of cyclooxygenase-2 (COX-2) and nuclear factor-kappa B (NF-kappaB) in nasal polyps co-cultured with clarithromycin, and to investigate their roles in CRS pathogenesis.
METHOD:Nasal polyps from 11 patients with chronic rhinosinusitis (CRS) were cultured for 24 hours with different doses of clarithromycin (0, 10(-6), 10(-5), 10(-4) mol/L). Western blot and real time fluorescence quantitative PCR were performed to detect the expressions of COX-2 and NF-kappaB subunits.
RESULT:The expression levels of COX-2, NF-kappaBp50 and NF-kappaBp65 were most high in control groups (0 mol/L clarithromycin). The expressions of COX-2, NF-kappaBp50 and NF-kappaBp65 were dose-dependently attenuated as the concentrations of clarithromycin increased. Significantly positive correlation between RNA expressions of COX-2 and NF-kappaB subunits in each CRS group was confirmed by Pearson correlation treatment (P<0.05).
CONCLUSION:The increased expression of COX-2 is involved in the inflammation in CRS. It indicate that clarithromycin may play an anti-inflammatory effect on CRS by decreasing the synthesis of COX-2 through blocking NF-kappaB pathway.
