Expression and significance of Survivin mRNA in xenotransplanted nasopharyngeal carcinoma treated by paclitaxel combined with radiotherapy.
- Author:
Shuai ZHANG
1
;
Jianyun XIAO
;
Suping ZHAO
;
Yuanzheng QIU
;
Yong LIU
;
Chenglong WANG
;
Yongquan TIAN
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China. zhang-shuaixn@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Brachytherapy;
Cell Line, Tumor;
Humans;
Inhibitor of Apoptosis Proteins;
genetics;
metabolism;
Mice;
Mice, Nude;
Nasopharyngeal Neoplasms;
metabolism;
therapy;
Paclitaxel;
therapeutic use;
RNA, Messenger;
genetics;
Repressor Proteins;
genetics;
metabolism;
Survivin;
Xenograft Model Antitumor Assays
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(17):796-799
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and significance of Survivin mRNA in xenotransplanted nasopharyngeal carcinoma treated by paclitaxel combined with radiotherapy.
METHOD:Xenotransplanted nasopharyngeal carcinoma was established by CNE-2 cell line, then grouped and treated with paclitaxel, radiotherapy, paclitaxel combined with radiotherapy respectively. Xenotransplanted tumor volume was measured; tumor specimens were confirmed by routine hemotoxylin-eosin staining; apoptosis index was assayed by flow cytometry and Survivin mRNA was detected by one step RT-PCR.
RESULT:Xenotransplanted tumor growth was significantly inhibited by paclitaxel combined with radiotherapy and its inhibition rate was 99.3%. Compared to control group, apoptosis index was apparently increased in the other three groups (P<0.05), especially in the combined therapy group (P<0.05). Survivin mRNA expression was obviously decreased in the combined therapy group (P<0.05); whereas there was no difference in its expression among the groups of paclitaxel, radiotherapy, and control group (P>0.05).
CONCLUSION:Paclitaxel combined with radiotherapy can induce significant killing effect in xenotransplanted nasopharyngeal carcinoma; paclitaxel can enhance the radiosensitivity of xenotransplanted nasopharyngeal carcinoma and its mechanism may rely on the down-regulation of Survivin expression.
