Synergistic lethal effects of cetuximab combined with chemotherapy and/or radiotherapy in laryngeal squamous carcinoma cells.
- Author:
Rui HAN
;
Hui HUANGFU
;
Wei GAO
;
Chunming ZHANG
;
Na WANG
;
Zhuo LI
;
Binquan WANG
- Publication Type:Journal Article
- MeSH:
Antibodies, Monoclonal, Humanized;
pharmacology;
Apoptosis;
drug effects;
radiation effects;
Cell Line, Tumor;
Cetuximab;
Cisplatin;
pharmacology;
Combined Modality Therapy;
Humans
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(24):1375-1380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the sensitivity of cetuximab induced apoptosis in laryngeal squamous carcinoma cells Hep-2, and to evaluate the synergistic killing effects and regulation mechanism of cetuximab alone or cetuximab in combination with chemotherapeutic agents (cisplatin) or radiation means on Hep-2 cells.
METHOD:To investigate the cytotoxicities of cetuximab, cisplatin and radiation, cell counting kit-8 (CCK-8) assay was used for the detection of cell growth inhibition ratio, and fluorescence activated cell sorter FACS for the apoptotic rate and cell cycle distribution.
RESULT:Cetuximab had inhibitive effect on Hep-2 cells within a certain range of concentration in a time- and dose-dependence manner. The inhibition concentration 50% (IC50) of cetuximab on Hep-2 cells for 24 h was 1 036.84 microg/ml. For application of cisplatin and radiation, the apoptotic rate of Hep-2 cell was higher by combining with cetuximab than their single or combined administration. Moreover, the cell cycle arrested at G0/G1 phase.
CONCLUSION:Laryngeal cancer Hep-2 cells was sensitive to the cetuximab induced apoptosis. Cetuximab combined with cisplatin and/or radiation can increase the antiproliferative effects on Hep-2 cells. These findings suggest the synergistic combination of cetuximab and cytotoxic agents was sequence depended.
