Aberrant expression of CyclinE and p27 in laryngeal squamous cell carcinoma and the clinical significance.
- Author:
Damin CHAI
;
Zhengqi BAO
;
Jianguo HU
;
Li MA
;
Zhenzhong FENG
;
Yisheng TAO
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
metabolism;
pathology;
Cyclin E;
metabolism;
Cyclin-Dependent Kinase Inhibitor p27;
metabolism;
Female;
Head and Neck Neoplasms;
metabolism;
pathology;
Humans;
Laryngeal Mucosa;
metabolism;
pathology;
Laryngeal Neoplasms;
metabolism;
pathology;
Lymphatic Metastasis;
Male;
Middle Aged;
Oncogene Proteins;
metabolism;
Prognosis;
Squamous Cell Carcinoma of Head and Neck;
Survival Rate
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(3):165-174
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore new hallmarks affecting the prognosis of patients with laryngeal squamous cell carcinoma (LSCC) via investigating the expression of CyclinE and p27 in LSCC tissues.
METHOD:The expression of CyclinE and p27 was detected via Elivision immunohistochemical staining in 160 LSCC tissues and 20 normal laryngeal tissues (NLT). The relationship between CyclinE/ p27 and LSCC/ NLT was analyzed via Log-rank analysis. The relationship of CyclinE and p27 protein was statistically analyzed by spearman correlation analysis. The relationship between CyclinE/p27 and clinical-pathology-factors of patients with LSCC, such as age, gender, tumor site, diameter, differentiation, lymph node metastasis and PTNM stage were analyzed by Chi-square test. The relationship between clinical-pathology-factors, CyclinE, p27 and overall survival time of patients with LSCC was analyzed via Cox multiplicity and Kaplan-Meier survival analysis. A significant difference was recognized by P<0.05.
RESULT:In LSCC the positive rates of CyclinE and p27 protein was 62.50% and 41.25% respectively (P<0.05). In NLT the positive rates of CyclinE and p27 protein was 35% and 70% respectively (P<0.05). The expression of CyclinE or p27 protein was closely correlated with lymph node metastasis, PTNM stage of patients with LSCC (P<0.05). The expression of CyclinE and p27 had no significant correlations with patients' gender, age and tumor site, diameter differentiation (P>0.05 for all). A negative correlation was found between the expression of CyclinE and p27 protein, r= -0.767(P<0.05). Kaplan-Meier survival analysis showed that the overall survival rate of patients with LSCC was 36.9% (P<0.05). The 5-year survival rate in positive group of CyclinE was 8%, in negative group was 80% (P<0.05). On the contrary, the 5-year survival rate of patients with LSCC in positive group of p27 protein was 77.27%, the rate was 5.32% in negative group (P<0.05). Cox multivariate regression analysis revealed that lymph node metastasis, PTNM stage, CyclinE and p27 were independent risk factors of prognosis for patients with LSCC.
CONCLUSION:It is the molecular basis underlying the development and invasion/ metastasis of LSCC that activation of CyclinE gene accompanying inactivation of p27 gene. It is very important of co-detecting CyclinE and p27 protein to predict the prognosis of patients with LSCC.
