Apoptosis inducing effect by TRAIL on human laryngeal squamous carcinoma Hep-2 cells.
- Author:
Hongchao YAO
1
;
Hui XIAO
;
Chao WANG
;
Ming LIU
;
Dejun JIN
Author Information
1. Department of Otolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China. yaoyaosworld@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Carcinoma, Squamous Cell;
pathology;
Cell Line, Tumor;
Humans;
Laryngeal Neoplasms;
pathology;
TNF-Related Apoptosis-Inducing Ligand;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2011;25(3):127-129
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the apoptosis inducing effect of tumor necrosis factor related apoptosis-ligand (TRAIL) on human laryngeal squamous carcinoma Hep-2 cells and its effect mechanism.
METHOD:The human laryngeal squamous carcinoma Hep-2 cell line was treated with different concentration of TRAIL in vitro. The inhibition ratio of tumor cells was determined by MTT colorimetric assay, the incidence of cell apoptosis was determined by flow cytometry method. The morphologic changes of laryngeal squamous carcinoma Hep-2 cell were observed with transmission electron microscope.
RESULT:In vitro, all the different concentrations of TRAIL inhibited laryngeal squamous carcinoma cell's growth. The inhibited growth ratio showed significant concentration-dependence. The concentrations for inducing apoptosis-ratio(TRAIL 1, 10, 100 microg/L) determined by flow cytometry was (11.49 +/- 0.36)%, (22.31 +/- 0. 82)%, (59.64 +/- 1.10)% respectively in the study group, and (3.13 +/- 0.12)% in the control group, which was significantly different between these two groups (P < 0. 01).
CONCLUSION:In vitro, TRAIL inhibited the growth of human laryngeal squamous carcinoma Hep-2 cells. The induced apoptosis of TRAIL shows significant concentration- independence. TRAIL inhibits the growth of human laryngeal squamous carcinoma Hep-2 cells trough inducing apoptosis.
