Pathway analysis of the differential expression genes of oligonucleotide microarray of airway allergic diseases using GenMAPP.
- Author:
Jinmei XUE
1
;
Changqing ZHAO
;
Aihua LIANG
Author Information
1. Department of Otolaryngology, the Second Hospital of Shanxi Medical University, Shanxi, 030001, China.
- Publication Type:Journal Article
- MeSH:
Asthma;
diagnosis;
genetics;
Chemokine CXCL12;
metabolism;
Computational Biology;
Gene Expression Profiling;
Humans;
Oligonucleotide Array Sequence Analysis;
methods;
Receptors, CXCR4;
metabolism;
Rhinitis, Allergic, Seasonal;
diagnosis;
genetics;
Software
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2011;25(8):371-373
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detected the mechanism of allergic rhinitis associated with asthma with bioinformatics methods.
METHOD:GenMAPP software was used to analyze the expression profile of nasal mucosa of seasonal allergic rhinitis(SAR) and SAR associated with asthma of oligonucleotide microarray (Affymetrix HG-U133-plus2). One the first step,of differentially expressed genes screening were done, then differential gene database retrieval was established, at last pathway analysis was performed.
RESULT:689 genes out of 47 000 analyzed transcripts of nasal mucosa of SAR associated with asthma were differentially expressed at least 4-fold, in which 233 genes were up regulated and 456 genes were down regulated. These differential expression genes participate in 69 bio-pathways, in which the interaction pathway between cytokine and cytokine receptor was most. Chemotactic factor CXCL12 and its receptor CXCR4 expressed in SAR associated with asthma patients were up-regulated predominantly, compared with that in SAR patients.
CONCLUSION:Multiple pathways were involved in the development of SAR and SAR complicated with asthma. The CXCL12/CXCR4 axis might play a main role in the allergic airway diseases.
