Experimental study on the impact of urban inhalable particles on allergic rhinitis of rats.
- Author:
Zhimin XING
1
;
Xiaoling LIU
;
Lisheng YU
;
Min WANG
Author Information
1. Department of Otorhinolaryngology, Peking University People's Hospital, Beijing 100044, China. Xing-zhimin@tom.com
- Publication Type:Journal Article
- MeSH:
Air Pollutants;
analysis;
Animals;
Disease Models, Animal;
Incidence;
Male;
Particulate Matter;
Rats;
Rats, Sprague-Dawley;
Rhinitis, Allergic, Perennial;
epidemiology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2011;25(11):506-509
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:In order to study the effect of inhalable particles on provocation of rat allergic rhinitis model and its possible mechanism.
METHOD:After basic sensitizing the experimental rats with OVA by intraperitoneal injection, three groups were provocated by solo OVA, PM10+OVA and solo PM10 through nasal cavity respectively. The control group was sensitized by NS instead of OVA, and then provocated by NS as the method of experimental groups. The symptoms of rat during the provocation were observed and recorded. Then, the rats were sacrificed 24 hours after the last provocation, and the expression of IL-4 in nasal mucosa were detected by immunohistochemical method.
RESULT:The groups of OVA and PM10+OVA both had provocated the obvious symptoms of allergic rhinitis, while the groups of PM10 and NS had the symptoms of scratching nose occasionally. By statistical analysis of the amount of IL-4 positive cells, there were significant differences between each experimental group and NS group, and there was significant difference between three experimental groups. The amount of IL-4 positive cells in PM10+OVA group was obviously higher than that in other groups.
CONCLUSION:In the period of provocation the rat allergic rhinitis, the inhalable particles play a synergic role with allergen, and it can aggravate the symptoms of allergic rhinitis.
