Expression and significance of HIF-1alpha and iNOS in human middle ear cholesteatoma epithelium.
- Author:
E ZHANG
1
;
Shusheng GONG
Author Information
1. Department of Otolaryngology, the First Hospital of Wuhan, Wuhan, 430022, China.
- Publication Type:Journal Article
- MeSH:
Cholesteatoma, Middle Ear;
metabolism;
pathology;
Epithelium;
metabolism;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
metabolism;
Nitric Oxide Synthase Type II;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2007;21(10):463-465
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of Hypoxia inducible factor-1alpha (HIF-1alpha) and inducible Nitric Oxide Synthase (iNOS) in cholesteatoma and approach the possible role of them in the formation and development of the middle ear cholesteatoma.
METHOD:Immunohistochemical SP method was used to examine the expression of HIF-1alpha and iNOS protein in 21 middle ear cholesteatoma specimens and 11 samples of normal external ear canal skin.
RESULT:In the 21 middle ear cholesteatoma, in epithelium tissue samples and 11 external ear channel's normal skin specimens, the expression index of HIF-1alpha was 52.49 +/- 13.80, 0.60 +/- 0.49, and that of iNOS was 92.05 +/- 27.84, 1.15 +/- 0.84 respectively. The HIF-1alpha and iNOS expression index of cholesteatoma epithelium was significantly higher than that of external ear channel's normal skin (P < 0.01). Moreover in the cholesteatoma epithelium, linear correlation analysis showed that iNOS protein was positively correlated with HIF-1alpha protein expression (r = 0.536, P < 0.05).
CONCLUSION:High density of HIF-1alpha and iNOS are found in the epithelium of cholesteatoma; Hypoxia and its relative factors HIF-1alpha,iNOS may play an important role in the formation and development of cholesteatoma.
