The expression and significance of HIF-1alpha and COX-2 in laryngeal squamous cell carcinoma.
- Author:
Shihua YIN
1
Author Information
1. Department of Otorhinolaryngology, the First Clinical College, Guangxi University of Medical Sciences, Nanning, 530021, China. shihuayin@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
genetics;
metabolism;
pathology;
Cyclooxygenase 2;
genetics;
Female;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
genetics;
Laryngeal Mucosa;
pathology;
Laryngeal Neoplasms;
genetics;
pathology;
Male;
Middle Aged;
Neoplasm Staging;
Prognosis;
RNA, Messenger;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2007;21(18):820-824
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the protein and mRNA expression of HIF-1alpha and COX-2 in laryngeal squamous carcinoma, and their correlation with clinicopathologic parameters.
METHOD:The protein and mRNA expression of HIF-1alpha and COX-2 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 76 patients were examined by immunohistochemistry and reverse transcription polymerase chain reaction.
RESULT:1) The protein positive expression rate of HIF-1alpha and COX-2 in tumor samples were significantly higher than that in normal tissues (P < 0.05); The protein positive expression rate of HIF-1alpha were higher in moderately and poorly differentiated tumors than in well differentiated tumors (P < 0.05); While the protein positive expression rate of COX-2 were not associated with T stage (P > 0.05); 2) The mRNA positive expression rate of HIF-1alpha and COX-2 were 67.11% and 75.00% respectively, their expression were significantly higher in tumors than that in normal tissues (P < 0.05). The mRNA expression of COX-2 were not detected in normal tissues. The mRNA positive expression rate of HIF-1alpha were associated with T stage, but were not associated with differentiated clinicopathologic stage. 3) The protein positive expression rate of HIF-1alpha were associated with that of COX-2.
CONCLUSION:HIF-1alpha and COX-2 could up regulate each other to promote the development and metastasis of laryngeal squamous carcinoma.
