The changes and significance of microsatellite loss of heterozygosity in laryngeal premalignant lesions and squamous cell carcinoma.
- Author:
Tao GUO
1
;
Jingwu SUN
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, the Affiliated Province Hospital, Anhui Medical University, Hefei, 230001, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Carcinoma, Squamous Cell;
genetics;
pathology;
Female;
Humans;
Laryngeal Neoplasms;
genetics;
pathology;
Loss of Heterozygosity;
Male;
Microsatellite Repeats;
Middle Aged;
Neoplasm Staging;
Precancerous Conditions;
genetics;
pathology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2007;21(18):831-833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the character and significance of the loss of heterozygosity (LOH) on chromosomes 3P, 9P and 17P in laryngeal premalignant and malignant lesions.
METHOD:Allelic deletions analysis was performed with 6 polymorphic markers (D3S1234, D9S171, D9S1748, D9S162, INFA and p53) using polymerase chain reaction-single sequence length polymorphism-silver staining in 49 laryngeal premalignant and malignant lesions.
RESULT:Allelic loss was seen in 3.70% of the six markers in patients with simple hyperplasias (HP), 10.81% with low grade dysplasia (LGD), 26.03% with high grade dysplasia (HGD) and 38.67% with laryngeal squamous cell carcinoma (LSCC). Significant differences was found between allelic loss and clinical pathological grades (X2 = 17.686, P < 0.01). The rate of LOH increased remarkably with the lesions progressed from HP to LSCC. D9S171showed the highest incidence of LOH (35.00%) on the six markers.
CONCLUSION:Microsatellite DNA LOHs occur in early stage of laryngeal carcinogenesis. Microsatellite markers may be useful in the early diagnosis for laryngeal premalignant lesions.
