Low dose of ouabain protects injury of spiral ganglion neurons in vitro.
- Author:
Yonghao WEI
1
;
Hongjun XIAO
;
Yuanming JIANG
;
Chen YANG
;
Na ZHENG
Author Information
1. Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Cell Survival;
drug effects;
Cells, Cultured;
Ouabain;
administration & dosage;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Spiral Ganglion;
drug effects;
pathology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the protective effect of low dose ouabain on injury of cultured spiral ganglion neurons evoked by trophic factors deprived and to explore the mechanism.
METHOD:Spiral ganglion neurons were cultured in vitro for 7 days, and then exposed to Neurobasal medium + B27 supplement, Neurobasal medium only or Neurobasal medium + 10 nmol/L ouabain, respectively. After 48 h exposed to different medium, spiral ganglion neurons were stained by FITC labeled Annexin-V and PI, then apoptosis index were calculated using fluorescent microscope and flow cytometry, respectively. In addition, spiral ganglion tissues were cultured for 48 h to evaluate dendrite growth of spiral ganglion neurons in each group. Immunocytochemistry were performed to detect the level of Bcl-2 in each group at 6 h and 12 h.
RESULT:Spiral ganglion neurons exposed to Neurobasal medium +10 nmol/L ouabain have a similar apoptosis index compare with that of Neurobasal medium + B27 supplement, but a much lower apoptosis index than that of Neurobasal medium only. In addition, dendrite growth of spiral ganglion neurons exposed to Neurobasal medium +10 nmol/L ouabain was much longer than that of Neurobasal medium only. Bcl-2 level increased in spiral ganglion neurons exposed to Neurobasal medium + 10 nmol/L ouabain at 6 h.
CONCLUSION:Low dose of ouabain protects injury of spiral ganglion neurons evoked by trophic factors deprived in vitro. This effect may mediated by increasing the level of Bcl-2.
