A study of spiral ganglion cells death pathway induced by hydrogen peroxide.
- Author:
Linghui LUO
1
;
Shusheng GONG
;
Pei CHEN
Author Information
1. Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Apoptosis;
drug effects;
Cell Death;
drug effects;
Cells, Cultured;
Hydrogen Peroxide;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Spiral Ganglion;
cytology;
drug effects
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(5):222-224
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To make clear the molecular pathways involved in hydrogen peroxide-induced spiral ganglion cells death.
METHOD:The spiral ganglion cells of the newly born rats were primary cultured. Then the SGCs were exposed to hydrogen peroxide for different concentrations (0, 100, 200, 500 micromol/L) and for different hours (2, 4, 6 h). Cell nucleic were stained simultaneously with the DNA binding dyes Hoechst 33258 and propidium iodide.
RESULT:At lower concentrations of hydrogen peroxide, apoptosis was the main reason for cell death. At higher concentrations of hydrogen peroxide, the cells died mainly by necrosis.
CONCLUSION:The effects of hydrogen peroxide are dose and time dependency. Reactive oxygen species may play a role as an early molecule signal in the mechanism of SGCs death.
