Microsatellite alternation in laryngeal squamous cell carcinomas.
- Author:
Feifei CHEN
1
;
Wei ZHU
;
Bing LIU
;
Hong YU
;
Yang RUAN
;
Yuanding ZHANG
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, the First Hospital of Medical College, Jilin University, Changchun, 130021, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
genetics;
pathology;
Genes, Tumor Suppressor;
Humans;
Laryngeal Neoplasms;
genetics;
pathology;
Loss of Heterozygosity;
Microsatellite Instability;
Neoplasm Staging
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(6):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the microsatellite instability and loss of heterozygosity in the pathogenic mechanism of laryngeal squamous cell carcinomas.
METHOD:Forty cases squamous cell carcinomas of larynx were analyzed by comparing tumorous tissues and normal tissues around with 3 microsatellite markers from chromosome 3, 5 and 11, using PCR and PGE-AgNO3 staining.
RESULT:Among the 40 cases of laryngeal squamous cell carcinomas, 87.5% (35/40) of samples showed microsatellite instability or loss of heterozygosity in one to three microsatellite markers. High frequent microsatellite abnormal occurred at D5S592, it was 70% (28/40). Then the mutation rate of D3s1228 was 52.5% (21/40).
CONCLUSION:Our study revealed that tumor suppressor genes nearby chromosome 3p14 and 5q23 regions related to the pathogenesis of squamous cell carcinomas of larynx. A correlation between microsatellite alternation and stage of the tumor were found in D3s1228 and D5s592 chromosome regions.
