Mutation and abnormal expression of FHIT gene in hypopharyngeal carcinoma.
- Author:
Hao WU
1
;
Xinyong LUAN
;
Xinliang PAN
;
Jianfei HUANG
;
Qiang WANG
;
Dapeng LEI
Author Information
1. Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, China. entwuhao@163.com
- Publication Type:Journal Article
- MeSH:
Acid Anhydride Hydrolases;
genetics;
Aged;
Aged, 80 and over;
Base Sequence;
Carcinoma, Squamous Cell;
genetics;
Exons;
Female;
Gene Deletion;
Humans;
Hypopharyngeal Neoplasms;
genetics;
Male;
Middle Aged;
Molecular Sequence Data;
Mutation;
Neoplasm Proteins;
genetics
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(6):245-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the mutation and abnormal expression of the FHIT gene in human hypopharyngeal carcinoma.
METHOD:Matched normal and cancerous tissues from 24 patients with hypopharyngeal squamous cell carcinoma were obtained immediately after surgery. Total RNA was extracted, the FHIT gene was detected by nested RT-PCR and DNA sequencing technology.
RESULT:Normal sized FHIT transcript was detected in 23 of the 24 cases of normal matched tissues. Aberrant FHIT transcripts were found in 9/24 (37.5%) cases in hypopharyngeal carcinoma. Aberrant FHIT transcripts rate of well-differentiated, moderately differentiated and poorly differentiated squamous cell carcinoma, was 28.6% (2/7), 50.0% (4/8) and 33.3% (3/9), respectively. There the carcinoma with FHIT aberrant transcripts was neither corresponding to histological grade (P>0.05) nor to lymphatic metastasis. The sequence analyses of the two aberrant cDNAs revealed absence of exon 8 and exon 7-9. All initial deletion were in conjunction of exons.
CONCLUSION:High deletion rate of the FHIT gene in Chinese hypopharyngeal squamous cell carcinoma suggested the FHIT gene, a candidate tumor suppressor gene at 3p14.2, plays an important role in the tumor carcinogenesis, development and progression of the tumor, and thus may become a new prognostic marker in hypopharyngeal carcinoma.
