Inhibitory effects of siRNA expression plasmid specific to protein kinase CK2alpha on human laryngeal carcinoma xenograft in nude mice.
- Author:
Jianting WANG
1
;
Shusheng GONG
;
Yingpeng LIU
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Casein Kinase II;
genetics;
Cell Line, Tumor;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Plasmids;
RNA, Small Interfering;
genetics;
Transfection;
Xenograft Model Antitumor Assays
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;23(6):267-270
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect on the expression of protein kinase CK2alpha and growth of human laryngeal carcinoma xenograft in nude mice by applying small interfering RNA (siRNA) specific to protein kinase CK2alpha.
METHOD:Human laryngeal carcinoma Hep-2 cells were implanted under the skin of nude mice. After the tumors grew to a definite size, the tumors were injected with siRNA expression plasmid specific to protein kinase CK2alpha. The weight and volume of subcutaneous tumors were measured. The expression level of protein kinase CK2alpha mRNA and protein of tumors were measured with reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical technique, respectively.
RESULT:Protein kinase CK2alpha mRNA and protein expressions were significantly decreased in tumors transfected with siRNA expression plasmid specific to protein kinase CK2alpha (P<0.05). The tumor grew slowly after transfected with siRNA expression plasmid specific to protein kinase CK2alpha (P<0.01).
CONCLUSION:The siRNA expression plasmid specific to protein kinase CK2alpha may suppress the growth and the protein kinase CK2alpha expression of subcutaneous tumors. RNA interfering technology may be a new strategy for the treatment laryngeal cancer.
