Effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
- Author:
Xue GAO
;
Yutuo ZHANG
;
Yantao LIN
;
Haifeng LI
;
Yunchao XIN
;
Xiaolei ZHANG
;
Yunpeng XU
;
Xiaoling SHANG
- Publication Type:Journal Article
- MeSH:
Ambroxol;
pharmacology;
Biofilms;
drug effects;
Child;
Child, Preschool;
Haemophilus influenzae;
drug effects;
Humans;
Microbial Sensitivity Tests
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(10):721-723
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a biofilm model of Haemophilus influenzae and observe the effect of ambroxol on biofilm of Haemophilus influenzae and bactericidal action.
METHOD:Thirty strains of Haemophilus influenzae were isolated from adenoids of children with adenoidal hypertrophy. Two strains which could build stronger biofilms was selected in a 96-well plate. The effect of ambroxol on biofilms were determined by crystal violet, and the structure of biofilms were observed by scanning electron microscope (SEM). The numbers of viable bacterial in biofilm after ambroxol treatmented determined by plate culture count.
RESULT:Through crystal violet assay, significant difference (P < 0.01) between the two group after treatment was found when ambroxol concentration reached at 0.25 mg/ml and 0.49 mg/ml. The biofilms was destroyed by SEM. Ambroxol had the positive effect on bacterial killing by plate culture count,and the effect was in a dose dependent.
CONCLUSION:Ambroxol could destroy the biofilm of Haemophilus influenzae, and had bactericidal function in vitro.
