The effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig.
- Author:
Ning AN
;
Shengwu SHI
- Publication Type:Journal Article
- MeSH:
Animals;
Cisplatin;
toxicity;
Cochlea;
drug effects;
metabolism;
Female;
Glycosides;
pharmacology;
Guinea Pigs;
Male;
Proto-Oncogene Proteins c-jun;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(10):731-734
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig.
METHOD:Thirty guinea pigs were randomly divided into control group (10), cisplatin group (10) and forsythiaside group (10). The ototoxicity model was done with intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days. Forsythiaside (25 mg/kg per day) was injected 30 min before cisplatin solution treated in guinea pigs of forsythiaside group for 7 consecutive days. The saline instead of cisplatin was injected in normal control group. The distortion product otoacoustic emission (DPOAE) was detected before animals were killed. The expression of c-jun in cochlea of guinea pigs was detected by western blotting. The expression of c-jun mRNA in cochlea of guinea pigs was detected by reverse transcriptase polymerase chain reaction (RT-PCR).
RESULT:DPOAE amplitudes in cisplatin group was significantly lower than in control group (P < 0.01). Compared with cisplatin group, DPOAE amplitudes in forsythiaside group was increased significantly (P < 0.05). The expression of c-jun protein and mRNA were significantly increased in cisplatin group than in control group (P < 0.01). Compared with cisplatin group, the expression of c-jun protein and mRNA were significantly decreased in forsythiaside group.
CONCLUSION:Forsythiaside can significantly reduce the side effects induced by cisplatin through down-regulating the expression of c-jun.
