The inhibited effect of matrine on human nasopharyngeal carcinoma CNE2 cells and the expression of apoptosis related gene Caspase in vitro.
- Author:
Mao XIE
;
Guangyao HE
;
Shujing SHI
;
Yikang LIU
;
Jun CHEN
;
Anzhou TANG
- Publication Type:Journal Article
- MeSH:
Alkaloids;
pharmacology;
Apoptosis;
drug effects;
Carcinoma;
Caspase 3;
Caspase 8;
Caspase 9;
Caspases;
metabolism;
Cell Line, Tumor;
Humans;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
enzymology;
pathology;
Quinolizines;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(9):589-592
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the inhibited effect of matrine on human nasopharyngeal carcinoma CNE2 cells and the expression of apoptosis-related gene Caspase-3 mRNA, Caspase-3, Caspase-8, Caspase-9 protein. And to explore the inhibiting effect of matrine on the apoptosis of human nasopharyngeal carcinoma CNE2 cells.
METHOD:In vitro experiments, MTT assay was used to detect the effect of matrine on CNE2 cell proliferation with different concentration. The expression levels of Caspase-3 were detected by real-time PCR. Western Blot was used to gauge the levels of Caspase-3, Caspase-8 and Caspase-9 protein.
RESULT:MTT results showed significant inhibitory action of matrine on CNE2 cells proliferation in does-dependent manner, which could up-regulate the expression of Caspase-3 mRNA and Caspase-3, Caspase-8, Caspase-9 protein in a dose-dependent manner.
CONCLUSION:Matrine could inhibit CNE2 cells proliferation in a does-dependent,that was closely related to the up-regulation of Caspase-3 mRNA and Caspase-3, Caspase-8 and Caspase-9 protein expression, and to the cascade reaction of Caspase.
