Src family kinases affect the expression of Nav1.1 in spiral ganglion neurons.
- Author:
Qingjiao ZENG
;
Huiying CHEN
;
Jiping SU
- Publication Type:Journal Article
- MeSH:
Animals;
Indoles;
pharmacology;
Male;
NAV1.1 Voltage-Gated Sodium Channel;
metabolism;
Neurons;
metabolism;
Pyrimidines;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Spiral Ganglion;
cytology;
Sulfonamides;
pharmacology;
src-Family Kinases;
antagonists & inhibitors;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(11):789-792
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigated the effects of Src family kinases on the expression of mRNA and protein of Nav1.1 in spiral ganglion neurons.
METHOD:RT-PCR and Western blot techniques respectively explored the level of expression of mRNA and protein of Nav1.1 in spiral ganglion neurons by Src family kinases inhibitor.
RESULT:An application of the inhibitor of Src family kinases which was PP2 (10 micromol/L) and SU6656 (2 micromol/L) gived rise to the mRNA decreasing to 26% +/- 0.8% and 36% +/- 1.5% respectively (P < 0.05), and protein reducing to 39% +/- 12.5% and 53% +/- 1.7% severally (P < 0.05).
CONCLUSION:Administration of the inhibitor of Src family kinases could decrease the expression of mRNA and protein of Nav1.1 in spiral ganglion neurons.
