Expression of IL-35, Epstein-Barr virus-induced gene 3 mRNA and IL-12A mRNA in peripheral blood of patients with allergic rhinitis.
- Author:
Jun WAN
;
Ying LUO
;
Chunping YANG
;
Jiali LIU
;
Yunyan DU
;
Kun WANG
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Case-Control Studies;
Child;
Female;
Humans;
Interleukin-12 Subunit p35;
blood;
Interleukins;
blood;
Male;
Middle Aged;
Minor Histocompatibility Antigens;
Rhinitis, Allergic;
blood;
Young Adult
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(13):952-954
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the expression of IL-35, Epstein-Barr virus-induced gene 3 (EBI3) mRNA and IL-12A mRNA in peripheral blood of patients with allergic rhinitis and its significance.
METHOD:Peripheral blood were collected from patients with allergic rhinitis (46 cases) and healthy human controls(30 cases). The level of IL-35 in serum was measured by enzyme-linked immunosorbent assay. The subunit EB13 and IL-12A of IL-35 mRNA expressions in peripheral blood mononuclear cell(PBMC) were detected by SYBR Green real-time quantitative PCR.
RESULT:IL-35 level in AR group (251.22 +/- 46.27) ng/L was significantly lower compared with that in the normal control group (382.17 +/- 25.41) ng/L, (P < 0.01). The mRNA expression level of EBI3 in the patients with allergic rhinitis was significantly lower than that in the normal control group (P < 0.01), AR group EB13 mRNA level was about half of that in the normal control group. But the mRNA expression level of IL-12A in the patients with allergic rhinitis has not significant difference with that in the normal control group (P > 0. 05).
CONCLUSION:The decrease of IL-35 and EBI3 mRNA in AR,indicated that IL-35 and EBl3 mRNA may play an important role in allergic rhinitis.
