The expression and regulation of Th17 cell in murine modal of allergic rhinitis.
- Author:
Min SHI
1
;
Hui HUANGFU
;
Chunming ZHANG
;
Qingli HUANG
;
Min ZHANG
;
Yangzi JIN
Author Information
1. Department of Otorhinolaryngology, Affiliated First Hospital of Shanxi Medical University, Taiyuan 030001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Disease Models, Animal;
Female;
Interferon-gamma;
immunology;
Interleukin-17;
immunology;
Interleukin-4;
immunology;
Male;
Mice;
Mice, Inbred BALB C;
Ovalbumin;
immunology;
Rhinitis;
immunology;
metabolism;
Th17 Cells;
immunology;
Th2 Cells;
immunology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2011;25(14):652-655
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the function of Th17 cells in allergic rhinitis,through comparing the symptoms, pathology and and the quantity of Th1, Th2 and Th17 cytokine in normal mice, allergic rhinitis mice and allergic rhinitis mice with IL-17 antibody application.
METHOD:Thirty BALB/c mice were randomly divided into three groups, control group, allergic rhinitis group, and therapy group. The allergic rhinitis model was induced by classical method with ovalbumin. The therapy group was treated with IL-17 antibody. The concentration of IL-17, IL-4 and IFN-gamma in serum was measured by enzyme-linked immunosorbent assay (ELISA). Nasal mucosal inflammation was evaluated by HE staining. The expression of RORgammat mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS:The expression level of IL-17, IL-4 and RORgammat mRNA in allergic rhinitis group were significantly higher than those of control group and IL-17 antibody treated group (P < 0.05). While the expression level of IFN-gamma in allergic rhinitis group were significantly was lower than those of control group and IL-17 antibody treated group (P < 0.05). The inflammation reaction in therapy group abated with nasal mucosal HE staining.
CONCLUSION:The large quantity of Th2, Th17 cells were found in allergic rhinitis. It might be associated with the pathogenesis of allergic rhinitis. The control of Th17 cells expression may be an effective way to treat allergic rhinitis.
