A preliminary study on the regulation mechanism of p38MAPK on MUC5AC in allergic rhinitis.
- Author:
Zhenlin WANG
1
;
Peng LI
;
Yuan LI
;
Qiuhang ZHANG
;
Qiuyi QU
;
Yan QI
Author Information
1. Department of Otolaryngology--Head and Neck Surgery, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Female;
Humans;
Male;
Middle Aged;
Mucin 5AC;
metabolism;
Nasal Mucosa;
metabolism;
Rhinitis, Allergic, Perennial;
metabolism;
Signal Transduction;
p38 Mitogen-Activated Protein Kinases;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2011;25(20):943-946
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the effect of p38 mitogen activated protein kinase (p38MAPK) and cyclooxygenase-2 (COX-2) on the expression of mucin5AC (MUC5AC) in human nasal mucosa induced by histamine in vitro, and to investigate the pathogenesis of mucus hypersecretion in allergic rhinitis (AR).
METHOD:Western blot was performed to detect the protein expressions of p38MAPK, COX-2 and MUC5AC in nasal mucosa induced by histamine or blocked by selective inhibitors of p38MAPK and COX-2 of different concentration gradient.
RESULT:Weak expressions of p38MAPK. COX-2 and MUC5AC were detected in normal nasal mucosa in vitro. The protein expressions of p38MAPK. COX-2 and MUC5AC increased in nasal mucosa induced by histamine in a dose-dependent manner. The histamine induced protein expressions of COX-2 and MUC5AC were dose-dependently attenuated by selective inhibitor of COX-2, namely NS-398. No apparent influence of NS-398 on the expression of p38MAPK was observed. The histamine induced protein expressions of p38MAPK, C()X-2 and MUCbAC dose-dependently decreased after nasal mucosa was treated by selective inhibitor of p38MAPK, namely SB203580. And no significant change of MUC5AC protein expression induced by NS-398 or SB203580 was observed.
CONCLUSION:Our findings indicated that the histamine-induced increased expression of MUC5AC by activated p38MAPK/COX-2 may be a possible pathogenesis of mucus hypersecretion in AR.
