Asian Sand Dust Enhances Allergen-Induced Th2 Allergic Inflammatory Changes and Mucin Production in BALB/c Mouse Lungs.
10.4168/aair.2012.4.4.206
- Author:
Il Gyu KANG
1
;
Joo Hyun JUNG
;
Seon Tae KIM
Author Information
1. Department of Otorhinolaryngology, Gil Hospital, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea. rhinokim2002@hanmail.net
- Publication Type:Original Article
- Keywords:
Asian sand dust;
airway inflammation;
mucin
- MeSH:
Animals;
Asian Continental Ancestry Group;
Bronchoalveolar Lavage Fluid;
Cell Count;
Dust;
Enzyme-Linked Immunosorbent Assay;
Eosine Yellowish-(YS);
Female;
Hematoxylin;
Humans;
Immunoglobulin E;
Immunoglobulins;
Inflammation;
Interferon-gamma;
Interleukin-13;
Interleukin-4;
Interleukin-5;
Interleukins;
Lung;
Mice;
Mucins;
Ovalbumin;
Silicon Dioxide;
Transforming Growth Factor alpha
- From:Allergy, Asthma & Immunology Research
2012;4(4):206-213
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice. METHODS: Forty BALB/c female mice were divided into four groups: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-gamma) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TGF-alpha) was conducted. RESULTS: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05). The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-alpha-positive cells were also higher in group 4 than in group 3 (P<0.05). CONCLUSIONS: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.
