Effects of sodium valproate on proliferation and apoptosis of Hep-2 cells and associated mechanism.
- Author:
Junhui ZHANG
1
;
Yulin ZHAO
;
Mingmin DONG
Author Information
1. The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Flow Cytometry;
Humans;
Inhibitor of Apoptosis Proteins;
metabolism;
Survivin;
Valproic Acid;
pharmacology
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(8):359-362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of sodium valproate (VPA) on proliferation and apoptosis of human laryngeal carcinoma Hep-2 cells and its associated mechanism.
METHOD:Methabenzthiazuron (MTT) was used to observe the proliferation of human laryngeal carcinoma Hep-2 cells treated with various concentrations of VPA at different times. Flow cytometry(FCM) and RT-PCR were used to measure the apoptosis rate and the expression of Survivin mRNA in the Hep-2 cells treated with VPA at 3 mmol/L for different times.
RESULT:VPA inhibited growth of Hep-2 cells in a dose-dependent and time-dependent manner (P < 0.01). The apoptosis rate increased after the treatment by VPA at 3 mmol/L. There were significant differences between different time groups (P < 0.01). The expression of Survivin mRNA of Hep-2 cells were decreased in a time dependent manner (3 mmol/L) (P < 0.01).
CONCLUSION:VPA have obvious growth inhibition and induction of apoptosis on human laryngeal cancer Hep-2 cells, its mechanism is related to decrease the expression of Survivin.
